Answer:
■Gene sequences would be used to make Probes for both the Southern and Northern blots.
■The probes will be used to view the presence of each gene with the use of isolated genomic DNA obtained from the isolated bacterium
■Each probes hybridized to the genome shows the pathway is isolated and point of the genes were involved in the substrate catabolism
■The carbon source in the isolate is derived from the substrate inducing the catabolic pathway as RNA determine transcripts present
■Probes hybridizing to the same sequences would be used to determine the gene activity for the pathway as seen in the southern one
■since all the genes present in the genome couldn't be identified, the northern would be important to work on
■Catabolic pathway is determined by the same genes. Hence, the need for gene/transcript probes to hybridize to the transcriptome.
Nutrients cross the membrane wall with the help of protein channels.
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In gram positive bacteria have a peptidoglycan layer outside of the cell wall. Gram negative bacteria have peptidoglycan between membranes.
The natural penicillin have activity against non beta lactamase producing gram - positive cocci, which includes such as - viridans streptococci, anaerobic streptococcus. Penicillin work indirectly bursting bacteria cell walls. It kills bacteria through binding of that betalactum ring.
Gram positive bacteria have a much thicker layer of peptidoglycan and lack of the protection of an outer membrane. This class , penicillin was first antibiotic to be used widely and prevents the final cross linking step. There are some more classes of penicillin like- natural , penicillin-stable, aminopenicillins and extended-spectrum penicillin.
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