Answer:
First, you need to retrieve the FASTA sequences of the already sequenced genes. After that, you should make a sequence alignment on a software like CLUSTALW (https://www.ebi.ac.uk/Tools/msa/clustalw2/) to seek for the conserved regions of the gene. Based on this regions, you will design your FISH probe, which for bacteria can start in 33 bp (Ming Tan <em>et al.</em>, 2019), but a 200 bp probe is a very suitable size. There are several companies that fabricates this custom probes, and sell all the reagents to perform the experiments. Having this, you will perform your FISH experiment to see which cells are actively degrading Toluene.
Explanation:
The shape of the beak can tell you the function of that particular type of beak, and then the habitat in which the bird lives. For example, a bird with a long, slender beak most likely gets its food from hard to reach crevices in the ground or trees, and a bird with a short, thick beak most likely gets its food from either cracking items open or killing prey, because the shortness of the beak shows that it does not need to reach small places.
B. Roots take up nutrients and water from the ground like the mouth takes in food and drink.
Hope this helps!!
I think the answer is:
spreading of the disease, might result in more health issues, deaths may be a result too