Answer: what is the major environmental factor limiting the number of autotrophs at great. Which abiotic factor has the least effect on the ability of aerobic organisms to live and, all living things that directly or indirectly affect the environment are known as. Which statement about the release of chemicals by willow trees is correct?

Explanation:

3 0

4 years ago

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What is the only way that carbon dioxide is removed from the carbon cycle?

Leokris [45]

Explanation:

Animals and plants need to get rid of carbon dioxide gas through a process called respiration. Carbon moves from fossil fuels to the atmosphere when fuels are burned.

8 0

3 years ago

What is the dilution factor if 2 μl of DNA sample is added to 498 μl of water (Dilution factor = Final volume / Aliquot volume)?

polet [3.4K]

Answer:

The correct answers are: Dilution factor = 250 and concentration of DNA in undiluted sample = 2.5 μg/μl.

Explanation:

Volume of DNA taken = 2 μl.
Volume of added water = 498 μl.
Total volume of the sample = 2 μl + 498 μl = 500 μl.
Dilution factor = $\frac{Final Volume}{Aliquot volume} = 500\div2 = 250.$
A₂₆₀ of the given sample is = 0.2.
We know that 50 μg/ml concentration of pure double stranded DNA gives an absorbance, A₂₆₀ = 1.
Again, Concentration of DNA(µg/ml) = $A_{260}\times \texrm{dilution factor}\times 50$ µg/ml.
= $0.2\times\ 250\times 50 = 2500$ μg/ml.
In order to convert it into μg/μl, we have to divide the above value by 1000, hence we get, $2500\div1000 =2.5$ μg/μl.