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Molodets [167]
3 years ago
14

Which of the following statements about manual Sanger sequencing is true? View Available Hint(s) Which of the following statemen

ts about manual Sanger sequencing is true? Each of the four terminating ddNTPs is labeled with a different fluorescent dye. The DNA sequence is read from the top of the gel to the bottom. One sequencing reaction is performed.
Biology
1 answer:
Fed [463]3 years ago
4 0

Answer:

One sequencing reaction is performed

Explanation:

The manual Sanger sequencing technique is based on the termination of DNA synthesis by the addition of a ddNTP, which impairs the full elongation of the molecule. Originally, you would perform four parallel reaction, one for each type of ddNTP (A, G, T, C). So in each reaction you would get sequences of different lengths, all finished with the ddNTP you added in that tube.

By running an electrophoresis for nucleotides, using a gel with enough resolution to separate the sequences by on base pair (bp). If you run each of the reaction mixture in a different rail, you should get fragments of the lengths corresponding to all the positions the nucleotide you added as a ddNTP occupy in the sequence.

Also, as the fragments are separated based on their molecular weight, so smaller ones migrate further in the gel. That means the gel should be read from bottom to top (note that the smaller fragments were terminated earlier than the larger ones).

For example, if in the rail of ddATP, you get a fragment of four bp, that means the fourth nucleotide of the sequence it's an A.

Per each reaction, only one sequence can be sequenced, otherwise it would be impossible to know which fragments correspond to the different sequences.

This method doesn't need any kind of dye to be used on the different ddNTPs as long as they are added in separate reactions.  

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What is the accumulation of differences between species or populations?
FromTheMoon [43]

Answer: Divergence evolution or divergent selection

Explanation:

The divergent evolution/divergent selection can be defined as the process of accumulation of differences between the related populations lies within the species. This process leads to speciation.

This occurs when the two populations of the same species get separated by a geographic barrier. The separated members of the same species experience different selective pressure that are responsible for adaptation in the new environment.

7 0
3 years ago
Which of the following are compounds? (Choose all that apply.)
STatiana [176]

Answer:

Water, organic biological molecules, sodium chloride, glucose.

Explanation:

A compound is anything composed of two or more separate elements.

  • O2 and H2 are a singular elements, and a chain of carbon atoms is multiple of the same element, so these are not considered compounds.
  • Sodium chloride is sodium and chlorine, and water is oxygen and hydrogen. Glucose is a compound of sugar, and organic biological (or biomolecules) are made up of carbon and hydrogen, so these are all considered compounds

I hope this helps! Happy learning, friend :)

8 0
3 years ago
A large group of seals from Prince Harold Coast Antarctica has emigrated to Oates Coast Antarctica How will this impact the rema
Ray Of Light [21]

Answer:

B

Explanation:

Because now it’s a larger group

5 0
3 years ago
Read 2 more answers
Do all animals need the same amount of each macromolecule?
dexar [7]

Answer:

The four main classes of organic compounds (carbohydrates, lipids, proteins, and nucleic acids) that are essential to the proper functioning of all living things are known as polymers or macromolecules. All of these compounds are built primarily of carbon, hydrogen, and oxygen but in different ratios.

Explanation:

plz mark me brilliantest

6 0
3 years ago
Green crabs (Carcinus maenas) are hyperosmotic regulators in brackish waters. When water salinity is lowered, a green crab respo
SashulF [63]

Answer:

Explanation:

Green crabs have chemoreceptors located on an <em>antenna</em>.

These are long segmented appendages near the crab´s eyes that have both chemoreceptors and allow them to feel the surroundings.

Crabs also have antennules, like short appendages close to the antennas that allow them to <em>sense</em> their environment. A crab can "taste" using hairs on their mouthparts, pincers and even its feet.

So they can detect the salinity of the water by "taste" using the antennules. If these antennules are bathed with nonsalty water, the information the crab´s brain receives is to increase the salinity of its body by the system explained above, increasing its rate of urine production.

I hope it helps!

7 0
3 years ago
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