Answer:
option A
Explanation:
In carrying out these experimental procedure, the same restriction enzyme that is used to cut out the gene of interest should also be the same used for the cutting of the plasmid to generate sticky ends. Then the desired gene is inserted into the cut plasmid then treatment with DNA ligase. Purified DNA ligase is utilized in gene cloning to join/ligate DNA molecules together forming a recombinant DNA. Thus, step 3 should be carried out after step 4.
<span>C.) A pea is a dicot, so the radicle grows and gives rise to a main root and its branches</span>
The answer to this question is B. Asp
So you know how it fits together, the ordering of the sequence.
Answer:
Thermosensitive liposomes (TSL) are promising tools used to deliver drugs to targeted region when local hyperthermia is applied (∼40–42°C) which triggers the membrane phase transformation from a solid gel-like state to a highly permeable liquid state. Selective lipid components have been used to in TSL formulations to increase plasma stability before hyperthermia and speed drug release rate after. Two generations of TSL technology have been developed. The traditional thermal sensitive liposomes (TTSL) have utilized DPPC and DSPC as a combination. The second generation, lysolipid thermally sensitive liposomes (LTSL) technology, has been developed with incorporation of lysolipids that form stabilized defects at phase transition temperature. LTSL maintains certain favorable attributes:
High percentage of lysolipids incorporation;
Minimum leakage for therapeutical drugs encapsulation;
Ultrafast drug release upon heating (3.5 times enhanced compared to TTSL). For example, ThermoDox, a commonly used LTSL drug for cancer, has been reported to release 100% of the encapsulated doxorubicin within 30s;
First and most successful formulation for intravascular drug release.
Explanation:
https://www.creative-biostructure.com/Lysolipid-Thermally-Sensitive-Liposomes-Production-612.htm