Type II restriction enzymes, such as Ecor I, that make staggered cuts within its recognition sequence, are considered more effective in biotechnology because they result in cohesive or sticky ends.
<h3>What is Ecor I and why are sticky ends important?</h3>
Ecor I is a kind of restriction enzyme which is obtained from Escherichia coli. The palindromic sequence recognized by this enzyme is 5' - GAATTC - 3'. It makes the following cuts between G and A on both the strands of the DNA to form sticky ends:
5' - G↓AATTC - 3'
3' - CTTAA↑G - 5'
Sticky ends are a fragment of DNA which is produced through a staggered cut, by the use of restriction enzyme. In this the terminal portion stretches with unpaired nucleotides. These kind of ends are easy to ligate when rDNA needs to be formed.
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cells in the vegetal half of the embryo should be unpigmented those in the animal half and there should be heavily pigmented.
Explanation:
The Yolk molecules moving in the vegetal halves of the egg which is the case in most of the animals, slows down the cell division. These lesser division area or the zone demarcates the area to be vegetal pole and due to lesser cell division, it becomes unpigmented or less pigmented Based on the observation time and perspect with assuming no movement.
The pole opposite to the vegetal, is animal pole which with absence or less of yolk, gets more ability to divide and hence become more pigmented or heavily pigmented. This brings polarity to the egg and becomes telolecithal egg.
The answer possibly could be a drought but I’m not 100%
Answer:
If the products contain 3 nitrogen atoms, then so did the reactants since overall mass is conserved in a chemical reaction.
Explanation: