Answer:
The missing piece of information needs to be added
■Was the cell number on plate D (containing 0.1 ml of cell dilution from tube D) ten-fold of those on plate E?
Not at all.
From our findings, the cells number of plate D was not tenfold to that on plate E.
The cells number from the result for plate D was 63 with plate E assumed to show 6.3 (approx. 6).
We can therefore say, we see mainly 3 colonies for plate E that was represented on the table which indicates that Tube D can't measure up to tenfold of E.
■What might be the reasons if the results did not demonstrate the relationship of ten-fold dilution (you have to answer this question even your results did demonstrate the relationship of ten-fold dilution)?
The reason why the results did not show the relationship of tenfold dilution is due to the tube not been mixed properly resulting to an unequal movement of culture to spread.
This could also be as an aftermath of spilling of liquid in the period of transfer or production of air bubbles that lowers the volume of culture moved to the plate for spreading.
