MycolicWhat are the components of acid alcohol?Prevent contamination of the smear by the stain pptWhat is the purpose of placing a filter paper on the glass slide?<span>1. Flood with carbolfuchsin. Heat until steam is given off for about 5 min
2. Allow to stand for more 5 min w/o heating. Remove the filter paper
3. Decolorize with acid alcohol while continously agitating the slide, then wash with water
4. Counterstain with methylen blue for 30 sec., wash with water then let it dry</span>Discuss the procedure in using ziehl-neelsen methodRedWhat is the color of an acid fast bacteria?Blue/greenWhat is the color of non-acid fast bacteria?<span>4g basic fuchsin
20 ml of 95% ethanol
8 ml phenol
100 ml distilled water</span>What are the components of kinyoun's carbolfuchsin?<span>1. Flood the smear with kinyoun's for about 5.min
2. Rinse with deionized water
3. Decolorize to faint pink color with acid alcohol for 3 min
4. Rinse again with deionized water
5. Flood with methylene blue for (4min), dry and examine under OIO</span>Discuss the procedure in using kinyoun's methodHorizontal or vertical scanningWhat is the proper way or direction of reading your stained smear?Horizontal scanningWhat is known as standard scanning?Pappenheims methodIn what method we use rosalic acid and alcohol as decolorizer?ColorlessWhat is the reaction of Mycobacterium smegmantis when rosalic acid and alcohol was added as decolorizer in pappenheims method?Diluted alcholic fuchsinWhat is use as stain i youe baumgarten's method?Red
Answer:
Considering the <u>whole progeny</u> (100%), there will be
- 25% Black male kittens, XBY
- 0% Calico Male kittens
- 25% Calico female kittens, XBXR
Explanation:
<u>Available data:</u>
- The gene for Calico (multicolored) cats is an x-linked trait and codominant
- Calico Females receive a "B" and an "R" gene, and have black and orange splotches on white coats. Their genotype is XBXR.
- Males can only be black or orange, but never calico. Their genotype is XBY and XRY
Cross: a female calico cat with a black male
Parentals) XBXR x XBY
Gametes) XB XR XB Y
Punnett square) XB XR
XB XBXB XBXR
Y XBY XRY
F1) Among the whole progeny:
- 2/4 = 50% will be black (female XBXB and male XBY)
- 1/4 = 25% will be Calico (female XBXR)
- 1/4 = 25% will be Orange (male XRY)
Among females:
- 1/2 = 50% of females will be black, XBXB
- 1/2 = 50% of females will be Calico, XBXR
Among males:
- 1/2 = 50% of males will be black, XBY
- 1/2 = 50% of males will be orange, XRY