Question

A binding protein binds to a ligand l with a kd of 400 nm. what is the concentration of ligand when y is (a) 0.25, (b) 0.6, (c) 0.95?

Answer 1

Hey there!:

The fractional saturation y is defined as :

y =  [ L ] / Kd + [ L ]

where :

[ L ] = concentration of binding ligand

Kd = 400 nm