Answer:
she can eat 0.4 hot dogs per minute
<h2>
Answer:</h2>
4.82 × 10⁻⁴
<h3>
Explanation:</h3>
Move the decimal so there is one non-zero digit to the left of the decimal point. The number of decimal places you move will be the exponent on the 10
. If the decimal is being moved to the right, the exponent will be negative. If the decimal is being moved to the left, the exponent will be positive.
Answer:
x=1 y=-9
Step-by-step explanation:
plug the given x and y values into the equation
y=-2(4)-1
y=-8-1
y=-9
1=-2x-1
+1 +1
2=-2x
/2 /2
1=x
I got 3x + 2 = 5x - 4 which is a three-step equation. Does that answer your question?
Cloning vectors are the DNA molecules that can carry a foreign DNA segment into the the host cell.The vectors used in recombinant DNA technology can be plasmids, cosmids, bacteriophages etc.
Plasmids: Self replicating, circular, extra chromosomal DNA present in bacteria. Plasmids have only one or two copies per cell.
Bacteriophages: Virus infecting bacteria. Bacteriophages have high number per cell, so their copy number is also high in genome.
Cosmids: Hybrid vectors derived from plasmids which contain cos site of lambda phage.
Feature required to facilitate cloning into vector: Origin of replication, Selectable marker, unique restriction sites.
The purpose of cloning vector is often to make numerous copies of the inserted gene.
Expression Vectors: The cloning vector containing suitable expression signals to have maximum gene expression, is called expression vector.
The following expression signals are introduced into gene cloned vectors to get maximum expression:
Insertion of a strong promoter.
Insertion of a strong termination codon.
Adjustment of distance between promoter and cloned gene.
Insertion of transcription termination sequence.
Insertion of a strong translation initiation sequence.
