Answer:
The two important conditions that must be met before gram staining are-
1. The age of the culture should not exceed 24 hours.
2. Over heating, decolonization should be avoided.
Explanation:
Gram staining is a technique developed to distinguish the bacteria on the basis of the composition of the cell wall around the bacteria.
The gram stain is a sensitive technique so must be performed carefully. The precautions should be taken to avoid any error while gram staining that is-
1. Age of the culture- The culture should be freshly prepared just 18-24 hours before the experiment as above 24 hours bacteria lose their ability to retain the color so one would not be able to differentiate between the cultures.
2. While performing the experiment one should avoid overheating and decolorization with ethanol as both the factors lead to the loss of crystal violet stain from the cell and they take pinkish color after safranin staying.
When we drinks water, those little droplets on the outside of the glass are condensation. I think?
Members of phylum Porifera are commonly known as spongs. They are generally marine and mostly asymmetrical animals. These are primitive multicellular animals and have cellular level of organization (lacks tissues). These Organisms (belonging to phylum Porifera) lacks most of the structures that are present in multicellular organisms like digestive system, respiratory system, Brain etc
Body of Spongs are made up of spicules or spongin fibers and these organism contain a water canal system which helps them in digestion, reproduction and catching their food in marine habitat.
<span>Is the study of humankind in all times and places.
-the study of all humans throughout time and space. Hope it helped!</span>
If D is dominant and d is recessive
1. Dd
2. DD
3. dd
