Answer:
a. time (on the x axis)
b. the product formed - molecules/minute (on the y axis)
Explanation:
The independent variable is the one you control while carrying out the experiment, you can adjust for it as needed and how much ever needed. The dependent variable is the one which you cannot control or vary at you own will during the experiment, it is essentially the data you collect from running the experiment.
Answer:
b. A second marker in the knock-out cassette, that if inserted into the genome results in cell death when plated on selective media.
Explanation:
General recombination, also known as homologous recombination, refers to the naturally occurring process of exchange of genetic material between pairs of homologous DNA sequences. This process (homologous recombination) can be exploited by genetic engineering to insert DNA segments of interest at target genes. Moreover, a cassette is a mobile DNA segment containing almost a gene and a recombination site, which is integrated into the <em>locus</em>/<em>loci</em> of interest by homologous recombination. A cassette may contain a DNA segment called 'negative marker' which prevents growth under particular conditions, while a positive marker permits growth under certain conditions. In consequence, a second marker consisting of a drug cassette may be used as a negative marker in order to evidence its insertion by inducing cell death when they are plated in selective conditions.
Answer:
cancellation
Explanation:
A factor in the numerator is <em>cancelled by dividing it</em> by the same factor in the denominator. The quotient is 1, the multiplicative identity element.
