Question

. You have constructed four different libraries: a genomic library made fromDNA isolated from human brain tissue, a genomic library made from DNAisolated from human muscle tissue, a human brain cDNA library, and a humanmuscle cDNA library.A) Which of these would have the greatest diversity of sequences?B) Would the sequences contained in each library be expected to overlapcompletely, partially, or not at all?

Answer 1

Answer:

A) Brain genomic library and muscle genomic library.

B) Brain genomic library and muscle genomic library - overlap completely.

Human brain cDNA library, and a human muscle cDNA library and other library is partially overlap.

Explanation:

A) The genomic library contains the whole genome content of the organism whereas cDNA library contains the coding genome of the organisms. Brain genomic library and muscle genomic library will constitute the all the genomic sequences of brain and muscle.  The cDNA library is prepared from the mRNA and the coding regions are present in this library.

B) The overlapping in the genome library might occur due to the common sequences present in the genome. Brain genomic library and muscle genomic library might completely overlap with each other as they have more sequence common among each other. All the other library may be partially overlap with each other as they have some common DNA sequences and neither library can have unique sequences.