The answer is D. This means that is you have a determined amount of force (in newtons) being applied on an object of known mass (in kilograms), then to determine the acceleration of the object, divide the force by the mass (or multiplying the force with the inverse of the mass of the object). This behavior of objects is stipulated in Newtons/ second law. Increasing the force increases the acceleration while increasing the mass decreases the acceleration of the object. Acceleration is a vector quantity meaning it has magnitude and direction.
Answer:
Given:
Number of colonies on plate = 40
Amount of DNA used for transformation = 100ng
Volume of competent cells used = 100ul
Total volume = 1ml (1000ul)
Volume plated for growth = 200ul
The amount of cells transformed per 1 µg of DNA is called the transformation efficiency
(CFU is colony forming units) => (number of colonies on plate/ng of DNA plated) X 1000 ng/µg = CFU/µg of DNA
Amount of DNA used for plating = 100ng * (200/1000)
= 100ng * (1/5)
= 100/5 = 20ng
Efficiency calculation = 40 colonies / 20ng = 2 colonies/ng
(2 colonies/ng) * (1000 ng/µg) = 2000 CFU/ug DNA
Transformation efficiency = 2000 Transformants / ug DNA
= 2 x 10³ Transformants / ug DNA
Explanation:
Efficiency of transformation is highest in the 100 pg-1 ng range, so Low transformation efficiency could be because of high amount of DNA used for transformation.
A.
<span>f(-2) = 2^(-2 - 2) </span>
<span>f(-2) = 2^(-4) </span>
<span>f(-2) = 1/2^4 </span>
<span>f(-2) = 1/16</span>
Energy flows from one animal or plant eating or getting eaten to the next anima above it. As for your translation, the crickets are eating the grass then are getting eaten by the secondary consumers and finally getting fed to the largest consumer and every time you go up in the food chain more and more energy is being passed on.
The answer is "because water is produced as a by-product"...the word dehydration should also be a major clue because that means water loss.
