Answer:
Abstract. DNA polymerase I (pol I) processes RNA primers during lagging-strand synthesis and fills small gaps during DNA repair reactions. However, it is unclear how pol I and pol III work together during replication and repair or how extensive pol I processing of Okazaki fragments is in vivo
Explanation:
Enzymes are not destroyed or altered in chemical reactions therefore can be used more that once.
In liquid media or broth difference between dry and wet will give biomass of the Epulopiscium.
Explanation:
Biomass of bacteria can be measured by dry or wet mass. Bacteria numbers can be counted by spread plate method under the microscope.
In solid media the colonies obtained are diluted and number of cells will be seen by plate count method or on automated cell counter. The number would help calculate biomass
The biomass will be calculated by measuring wet and dry mass. Equipments required will be:
hydraulic gravity convection oven and centrifugation set up.
A cellulose acetate filter membrane is used which is 47 mm in diameter and 0.45 micron of pore size.
The cells grown settles down due to gravity. They are stirred to evenly spread in the broth and is kept in centrifuge.
The cells obtained after centrifugation will be taken and wet weight is obtained.
To obtain dry weight the cells are placed in oven for 6 hours to 24 hours. The resultant cells are weighed and dry weight obtained.
Biomass will be calculated by subtracting the wet mass to dry mass. This way biomass is obtained in epulopisicuium. Here the cells will be of different size so on centrifugation 2 or more phases of cells can be seen due to gravity change.
