Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
Answer:
Tropical dry Climate: The weather is sunny pretty much all year. The ocean + the sun allows a tropical place to be great for growing food. Things such as coconuts and mangoes can be found. Its a great place to vacate because of the sunny climate, also clients will fall in LOVE with the warm beaches. Some activities would be to surf, sunbathe, and study the natrual habitat. The animals are also very interesting. Monkeys and chameleons would be great temporary pets to take care of.
I hope this was good enough, god bless you!!
Oxygen, liquid water, and life is on planet earth
Answer:
1. interphase is the portion of the cell cycle that is not accompanied by gross changes under the microscope while phases is a distinct period or stage in a series of events or a process of change our development.
