Answer:
Given:
Number of colonies on plate = 40
Amount of DNA used for transformation = 100ng
Volume of competent cells used = 100ul
Total volume = 1ml (1000ul)
Volume plated for growth = 200ul
The amount of cells transformed per 1 µg of DNA is called the transformation efficiency
(CFU is colony forming units) => (number of colonies on plate/ng of DNA plated) X 1000 ng/µg = CFU/µg of DNA
Amount of DNA used for plating = 100ng * (200/1000)
= 100ng * (1/5)
= 100/5 = 20ng
Efficiency calculation = 40 colonies / 20ng = 2 colonies/ng
(2 colonies/ng) * (1000 ng/µg) = 2000 CFU/ug DNA
Transformation efficiency = 2000 Transformants / ug DNA
= 2 x 10³ Transformants / ug DNA
Explanation:
Efficiency of transformation is highest in the 100 pg-1 ng range, so Low transformation efficiency could be because of high amount of DNA used for transformation.
Natural Selection could produce new variations of body parts.
Answer:
all except green
Explanation:
all except green because the plant is reflecting green light, not absorbing it :)
The answer is oxygen
hope it helps!
Answer:
The history of the human evolution can be studied by the fossil and DNA evidence. The similarities and advancement in certain characters has revealed the human evolution.
Different evidences like change in the diet, locomotion, brain size, walking ability as compared with the present species and species appeared 6 million years ago. The human genetics evidence and different paintings explain the culture of earlier homo species.The H. sapiens species has highly advance character that help them to survive than any other hominid species. The neanderthals extinction might occur due to their aggressive behavior, low social ability and their large cranial size.