It is because of what we called Greenhouse Gases. Greenhouse Gases absorbs the heat of the sun and brings it out to the earth. The heat of the sun travels 8 minutes and 20 seconds.
<span>The water diffuses out of the cell to equalize the solute concentrations
Salt is too big to pass through the cell and water is the only so water tries to "balance" the concentration on the inside and outside.
</span>
Answer:
a) k_m = 4.08 uM
V_{max} = 20.07 uM/min
b) k_m = 8.16 uM
Explanation:
Given that:
For Enzyme A:
the substrate concentration [S] = 40 uM
the initial velocity rate v = 10 uM/min
when it was 4mM, v = 20 uM/min
i.e.
at 4mM = 4000 uM;
Using Michealis -menten equation;
when v = 10
![V = \dfrac{V_{max}[S]}{k_m+[S]}](https://tex.z-dn.net/?f=V%20%3D%20%5Cdfrac%7BV_%7Bmax%7D%5BS%5D%7D%7Bk_m%2B%5BS%5D%7D)
∴



when v= 20



equating equation (1) and (2):


let multiply equation (1) by 100 and equation (2) by 1
4000V_{max} - 1000K_m = 4000
<u>4000V_{max} - 20 k_m = 8000 </u>
0 -980k_m = 4000
k_m = 4000/-980
k_m = 4.08 uM
replacing the value of k_m into equation (1)
40{V_max } - 10(4.08) = 400
40{V_max } - 40.8 = 400
40{V_max } = 400 + 40.8
40{V_max } = 440.8
V_{max} = 440.8/40
V_{max} = 11.02 uM/min
b)
Since V_{max} of A ie equivalent to that of B; then:
V_{max} of B = 11.02 uM/min
Here;
[S] = 80 uM
V = 10 uM/min
∴

10(k_m +80) = 881.6
10k_m = 881.6 - 800
10k_m = 81.6
k_m = 81.6/10
k_m = 8.16 uM