Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
Answer:
An example of a slow change is erosion and an example of rapid change is an earthquake.
Answer:
Since every person inherited DNA from their parents, who inherited it from their parents, and so on, a person's DNA is made up of the DNA of their ancestors. If you and another person both have the same ancestor, there's a chance that you both inherited some of the same DNA.
Explanation:
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Thirty-five percent<span> of the trees cut down are used to make paper. That means sixty-</span>five percent<span> of the trees cut down are used for something other than paper. Thank you for posting your question. I hope this answer helped you.</span>
Answer:
Insulin > Glucagon.
Explanation:
The blood glucose level in the body is maintained by the two hormones known as insulin and glucagon. These hormones are released by the beta cells and alpha cells of the pancreas.
The insulin decreases the blood glucose level whereas glucagon increase the blood glucose level. The individual is hypoglycemic means that he has low blood glucose level in his body. At this condition, the body has high insulin and low glucagon level in the body.
Thus, the answer is Insulin > Glucagon.
