Answer:
DNA ligase
Explanation:
DNA replication is a process whereby a particular DNA makes a copy of or synthesizes itself. It consists of several steps with some important enzymes for successful, error free replication. The various steps are as follows;
- Unwinding of the double helix structure of the DNA and formation of replication fork. The enzyme involved here is the DNA helicase.
- Primer, a short piece of RNA becomes synthesized and binds to the 3' end of one of the 2 strands of the DNA, the leading strand. The enzyme involved is the DNA primase.
- Replication of the <em>leading strand</em> then proceeds with the elongation of the primer through the addition of bases in the 5' to 3' direction leading to the formation of continuous strands.
- The other strand of the DNA, known as the <em>lagging strand </em>starts its own replication by binding with multiple primers at different regions of the strand. Bases are then added to each primer leading to the formation of several, short discontinuous DNA strands known as Okazaki fragments. The enzyme involved in the elongation process is the DNA polymerase.
- Next is the termination of the replication process after the formation of the continuous and discontinuous strands. Exonuclease enzyme removes primers from the synthesized strands. Primers are replaced by appropriate DNA bases and the Okazaki fragments are joined to form a unified DNA strand by an enzyme known as the DNA ligase.
The many, few nucleotides long DNA segments observed by the Biochemist are the Okazaki fragments that should have been joined together by the DNA ligase.
Hence, DNA ligase must have been the component left of the mixture.
Answer:
Rays having same endpoint E but not in the opposite directions are ED and EC. This pair of rays meet at point E having different directions.
Explanation:
Mitochondria are cell organelles that are found in all human body cells except mature red blood cells. These organelles with a double membrane are the location in which cellular respiration occurs.
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Answer:
C.Both Gram positive and Gram negative cells would appear purple.
Explanation:
Gram staining is a technique used to differentiate bacteria to two main groups based on their difference in cell wall components.
Crystal violet, decolorizer(ethanol) and a counter stain are used.
Gram positive bacteria have a thicker peptidoglycan layer hence they will retain the crystal Violet stain even after decolorization. They will stain purple
Gram negative will stain pink due to losing the initial stain and taking up the counter stain after decolorization.
Therefore, if water is used instead of ethanol. Both bacteria will retain crystal violet stain in their cell wall.