Answer:
The C. elegans embryo is a powerful model system for studying the mechanics of metazoan cell division. Its primary advantage is that the architecture of the syncytial gonad makes it possible to use RNAi to generate oocytes whose cytoplasm is reproducibly (typically >95%) depleted of targeted essential gene products via a process that does not depend exclusively on intrinsic protein turnover. The depleted oocytes can then be analyzed as they attempt their first mitotic division following fertilization. Here we outline the characteristics that contribute to the usefulness of the C. elegans embryo for cell division studies. We provide a timeline for the first embryonic mitosis and highlight some of its key features. We also summarize some of the recent discoveries made using this system, particularly in the areas of nuclear envelope assembly/ dissassembly, centrosome dynamics, formation of the mitotic spindle, kinetochore assembly, chromosome segregation, and cytokinesis.
1. The C. elegans embryo as a system to study cell division
The C. elegans embryo is a powerful model system for studying the mechanics of metazoan cell division. Its primary advantage is that the syncytial gonad makes it possible to use RNA interference (RNAi) to generate oocytes whose cytoplasm is reproducibly (>95%) depleted of targeted essential gene products. Introduction of dsRNA rapidly catalyzes the destruction of the corresponding mRNA in many different systems. However, depletion of pre-existing protein is generally a slow process that depends on the half-life of the targeted protein. In contrast, in the C. elegans gonad, the protein present when the dsRNA is introduced is depleted by the continual packaging of maternal cytoplasm into oocytes (Figure 1). Since depletion relies on the rate of embryo production instead of protein half-life, the kinetics tend to be similar for different targets. By 36-48 hours after introduction of the dsRNA, newly formed oocytes are typically >95% depleted of the target protein.
Explanation:
Answer:
pioneer species
Explanation:
Primary succession begins in barren areas, such as on bare rock exposed by a retreating glacier. The first inhabitants are lichens or plants—those that can survive in such an environment. Over hundreds of years these “pioneer species” convert the rock into soil that can support plants.
Biological systems do not contradict the second law of thermodynamics. Even in this case, entropy is still always increasing. Biological systems can only decrease their own entropy by using copious amounts of energy and by increasing entropy in their surrounding environment. Also, unfavorable anabolism reactions are always paired with more favorable reactions, such as the use of ATP in order to make the overall Gibb's free energy of the reaction negative.
Explanation:
(1) The conglomerate contains some nonsedimentary rock fragments. (2) The conglomerate was formed from material that was buried deep underground. (3) The conglomerate's pebbles are all weathering at the same rate.
