V1/T1 = V2/T2
Substitute the value use ratio and proportion. Use calculator.
V1 = (V2 x T1) / T2
1 is initial, 2 is final
Answer:
Protein Concentration is 2.82mg/L
Explanation:
According to Beer-Lambert's Law, Absorbance is directly proportional to the concentration.
However, the concentration of a solution can be determined from a calibration curve, in which Absorbance is plotted on the y-axis and the Concentration on the x-axis.
Plotting the best line, the equation of line is used
y = mx + c
where y is absorbance = 0.150
m is slope = 0.0163
x is concentration
c is intercept = 0.104
inserting the values from the question
y = mx + c
0.150 = 0.0163x + 0.104
0.0163x = 0.150 - 0.104
0.0163x = 0.046
Divide both sides by 0.0163
0.0163x/0.0163 = 0.046/0.0163
x = 2.82
Concentration of protein = 2.82 mg/L
