2. Process to test hypotheses
<span>When the lid for a vat
of wine is slightly open, the yeast inside can continue to perform alcoholic
fermentation because the carbon dioxide produced by the yeast during
fermentation will act as a blanket over the wine. Nevertheless, the air around the fermenter
must be still and enough carbon dioxide should be produced to continue
alcoholic fermentation. However, at some point, carbon dioxide will fall and
can no longer protect the wine. This is the right time to get your wine covered
before it will be vulnerable to undesirable microorganisms. </span>
Therefore, open fermentation
is good in the early stages of fermentation because yeast need oxygen to synthesize sterols and assimilate
fatty acids to ensure the nutrients it needs to multiply and ferment the 70% of the sugar from the fruit. Furthermore,
the yeast is responsible in decomposing sugar from the grapes into alcohol and
carbon dioxide.
Black death, also known as the bubonic plague was one of the worst pandemics that ever happened, killing around 1/3 of Europe's population. It spread to Europe in the mid 1300's from China.
Black death victims were treated through magic, witchcraft, prayer and other methods. As for the two other options, they used medicine, but not antibiotics and radiation hadn't been discovered yet (it was discovered in the 1900's)
The answer is D. For producing recombinant, first you need to cut the insert part and vector part to get the same end by using restriction enzyme. Then using DNA ligase to bind these two parts to get a new plasmids.