A poison added to an in vitro translation mixture containing mRNA molecules with the sequence 5′-AUGAAAAAAAAAAAAUAA-3′ has the f
ollowing effect: the only product made is a Met-Lys dipeptide that remains attached to the ribosome. What is the most likely way in which the poison acts to inhibit protein synthesis? a. It inhibits binding of the small subunit of the ribosome to mRNA.b. It inhibits peptidyl transferase activity.c. It inhibits movement of the small subunit relative to the large subunit.d. It inhibits release factor. e. It mimics release factor.
The correct answer is c it inhibits the movement of small subunit relative to the large subunit.
Explanation:
According to the question the the poison that is added in vitro translation mixture containing mRNA molecules specific sequence result in formation of Met-lys dipeptide that remains attached to the ribosome.
This means the translocation step that deals with movement of smaller subunit of ribosome to the next codon(Here to the third codon) is blocked.
