When calibrating a spectrophotometer, measuring absorbance concurrently is the best option for a blank since it is proportional to the concentration.
Beer's law states that A = a b c, wherein there is the diffusion coefficient at a constant, b is the actual route length, & c is the concentration. Direct proportionality exists between b and c and absorbance.
Once the route length is doubled, incident light contacts double as many molecules in the solution. The consequence is a doubling of absorbance, which is equivalent to a doubling of molecule concentration.
There are two ways to detect chemicals using spectrum scanning. One approach involves turning the monochromator continuously with a stepping motor while gradually altering the wavelength connected to the output slit.
It is more practical to use diode array detectors. Up to a few hundred photodiodes may be incorporated into the chip that makes up this device.
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Answer:
hemoglobin will bind more oxygen when the partial pressure is low than when the partial pressure is high.
Explanation:
Binding of hemoglobin to oxygen is regulated by several factors. However, the partial pressure of oxygen is the most important factor that determines how much oxygen will bind to hemoglobin. When the partial pressure of O2 is high, hemoglobin binds with large amounts of O2. On the other hand, when the partial pressure of O2 is low, hemoglobin is only partially saturated.
Therefore, the greater the partial pressure of oxygen, the more O2 will bind to hemoglobin until saturation is reached. This is why a lot of O2 binds to hemoglobin in pulmonary capillaries where the partial pressure of oxygen is high.
