Question

You perform an ELISA to test hospital soap samples for contamination with Pseudomonas aeruginosa. Other than the different molecule of interest, this ELISA works exactly like the one you saw in the video. You run the soap sample, a negative control, and a positive control, all in duplicate. How would you interpret the following results?a) The soap is POSITIVE for the Pseudomonas aeruginosa antigenb) You cannot interpret the soap data, because the POSITIVE control didn’t work.c) The soap is NEGATIVE for the Pseudomonas aeruginosa antigen.d) You cannot interpret the soap data, because the NEGATIVE control didn’t work.

Answer 1

Answer:

D) You cannot interpret the soap data, because the negative control didn't work.

Explanation:

ELISA or enzyme-linked immunosorbent essay is a technique used for the detection and quantification of proteins, enzymes, hormones and antibodies in a sample which is performed on the ELISA plate.

Here the in the above scenario there  are 8 wells in which sample (soap), negative and positive control are performed in duplicate.

At the result the negative control in one well, it is negative but in other well it is positive which shows that the test in invalid. And we cannot interpret on the basis of these test results because for our test validity both negative control samples should be negative.