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Rom4ik [11]
3 years ago
15

What causes ocean tides

Biology
1 answer:
butalik [34]3 years ago
3 0

Tides are the rise and fall of the oceans. They are caused by the gravity, or pull, of the Moon and Sun. The Moon's gravity is the main force in causing tides. It makes the oceans bulge out toward it. Another bulge occurs on the opposite side, because Earth is being pulled toward the Moon and away from the water. The water on the side farthest away from the Moon is least affected by its gravity.

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One of your lab partners has followed the recommended procedure of running Gram-positive and Gram-negative control organisms on
andreyandreev [35.5K]

Answer:

Reduced in holding time of decolrization step and also used less Alcohol because decolrization step is important in Gram's staining.The decolorization step must be performed carefully. Otherwise over-decolorization may occur. This step is critical and must be timed correctly otherwise the CV stain will be removed from the Gram-positive cells. If the decolorizing agent is applied on the cell for too long time, the Gram-positive organisms to appear Gram-negative..

Explanation:

Gram' staining is a technique used in microbiology labs to differentiate between Gram's positive and negative

<u>Gram-positive bacteria :</u>Stain dark purple due to retaining the primary dye called CV in the cell wall.

<u>:Gram-negative bacteria </u>Stain red or pink due to retaining the counter staining dye called Safranin or neutral red.

There are four basic step in Gram" staining

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2)Addition of Gram's Iodine

3)<u>Decolorization with 95% Ethyl Alcohol:</u>Alcohol or acetone dissolves the lipid outer membrane of Gram-negative bacteria, thus leaving the peptidoglycan layer exposed and increases the porosity of the cell wall. The CV-I complex is then washed away from the thin peptidoglycan layer, leaving Gram-negative bacteria colorless.

On the other hand, alcohol has a dehydrating effect on the cell walls of Gram-positive bacteria that causes the pores of the cell wall to shrink. The CV-I complex gets tightly bound into the multi-layered, highly cross-linked Gram-positive cell wall thus staining the cells purple.

The decolorization step must be performed carefully. Otherwise over-decolorization may occur. This step is critical and must be timed correctly otherwise the CV stain will be removed from the Gram-positive cells. If the decolorizing agent is applied on the cell for too long time, the Gram-positive organisms to appear Gram-negative. Under-decolorization occurs when the alcohol is not left on long enough to wash out the CV-I complex from the Gram-negative cells, resulting in Gram-negative bacteria to appear Gram-positive.

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Answer:

They are called covalent bonds.

Explanation:

I just went through the lesson.

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Explanation:

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