The correct answers are: the insertion of a DNA sequence into the targeted gene and deletions of the targeted gene
Cas9 is an enzyme endonuclease (which means it cuts nucleotides) that is part of the CRISPR-Cas9 complex- genome editing tool that can remove, add or alter sections of the DNA sequence.
Cas9 recognize the sequence that is going to be cut thanks to guide RNA- pre-designed RNA sequence complementary to sequence of interest. DNA repair machinery will try to fix the “damage” but it can add (insert) or delete some nucleotides.
 
        
             
        
        
        
Answer:
<h3><u>Required Answer</u><u>:</u><u>-</u></h3>
The intensification of agriculture has caused dramatic declines in farmland biodiversity (Carvalheiro et al., 2013; Senapathi et al., 2015). Since the 1990s, agricultural policies have been developed in Europe to mitigate this loss through agri-environmental schemes (AES). One AES is “sown wildflower strips”, the aim of which is to create new ecological infrastructures by sowing attractive wild flowers on arable land (a few % of the cultivated area). These ecological infrastructures fall within our definition of MIMS since they represent a massive introduction of managed species in the landscape.
 
        
             
        
        
        
This statement is true if that is the question
        
             
        
        
        
Runoff is the stage of hydrologic cycle