Metaphase is defined as the point at which all of the chromosome pairs are fully condensed, attached to the mitotic spindle, and aligned at the center—termed the metaphase plate.
What are the steps of metaphase?
- The chromosomes are all lined up at the center of the cell by the spindle during metaphase and are ready to divide.
- At the metaphase plate, all the chromosomes line up (not a physical structure, just a term for the plane where the chromosomes line up).
- The two kinetochores of each chromosome should be joined to microtubules coming from opposing spindle poles at this point.
- The cell will verify that all the chromosomes are at the metaphase plate and that their kinetochores are properly linked to microtubules before moving on to anaphase.
- The sister chromatids will split equally between the two daughter cells when the two cells separate in the following phase thanks to the spindle checkpoint, which is where it happens.
- A chromosome that is misaligned or improperly connected will prevent the cell from dividing unless the issue is resolved.
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