1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
levacccp [35]
3 years ago
7

While performing the acid-fast stain procedure, you heat your fixed bacteria with carbolfuchsin for 5 minutes, let the slide coo

l, and then rinse with water. what is your next step?
Biology
2 answers:
Lilit [14]3 years ago
5 0
In the acid-fast or Ziehl-Neelsen stain, following the referred washing, the slide should be covered in 1%-3% acid alcohol to decolourise until the smear turns into light pink. After that, wash well with clean water to stop the decolourisation.
allochka39001 [22]3 years ago
3 0

I believe the correct answer is add secondary stain

<h2>Explanation:</h2>

The Ziehl-Neelsen stain (ZN stain), also called the hot method of AFB staining, is a type of differential bacteriological stain used to identify acid-fast organisms, mainly Mycobacteria. Acid fast organisms are those which are capable of retaining the primary stain when treated with an acid (fast=holding capacity). Members of the Actinomycetes, genus Nocardia (N. brasiliensis and N. asteroides are opportunistic pathogens) are partially acid-fast.

<h2>Further Explanation:</h2>

Make a thin smear of the material for study and heat fix by passing the slide 3-4 times through the flame of a Bunsen burner or use a slide warmer at 65-75 C. Do not overheat.

Place the slide on staining rack and pour carbol fuschin over smear and heat gently underside of the slide by passing a flame under the rack until fumes appear (without boiling!). Do not overheat and allow it to stand for 5 minutes.

Rinse smears with water until no color appears in the effluent.

Pour 20% sulphuric acid, wait for one minute and keep on repeating this step until the slide appears light pink in color (15-20 sec).

Wash well with clean water.

Cover the smear with methylene blue or malachite green stain for 1–2 minutes.

Wash off the stain with clean water.

Wipe the back of the slide clean, and place it in a draining rack for the smear to air-dry (do not blot dry).

Examine the smear microscopically, using the 100x oil immersion objective.

The phenolic compound carbol fuchsin is used as the primary stain because it is lipid soluble and penetrates the waxy cell wall. Staining by carbol fuchsin is further enhanced by steam heating the preparation to melt the wax and allow the stain to move into the cell. Acid is used to decolorize nonacid-fast cells; acid-fast cells resist this decolorization. The ability of the bacteria to resist decolorization with acid confers acid -fastness to the bacterium. Following decolorization, the smear is counterstained with malachite green or methylene blue which stains the background material, providing a contrast colour against which the red AFB can be seen.

Acid alcohol can also be used as decolorizing solution, resistant organisms are referred to as Acid Fast Bacilli (AFB) or Acid Alcohol Fast Bacilli (AAFB).

Level: College

Subect: Microbiology

Topic: Bacteriology

You might be interested in
A student placed a disk of filter paper in each of the following solutions: disinfectant 1, disinfectant 2, disinfectant 3, and
beks73 [17]
<span>d. Disk soaked in distilled water

Experimentation is a scientific process wherein you test and verify a hypothesis based on its assumption is going to be supported or negated (null hypothesis). Hence experimentation involves the independent and dependent variable, also control variable as a level in the IV. </span>
8 0
3 years ago
If the sperm gamete that fertilizes an egg has a(n) ___ cheomosome, it will form female offspring.
Sophie [7]
If the sperm gametes that fertilizes an egg has an XX chromosomes, it will form female offspring. X is a female, XY is a female, Y is a male and XX is a female.
6 0
4 years ago
4. There is a new viral disease in the human population. You found that the
nordsb [41]

Answer:

COVI-19

Explanation:

3 0
3 years ago
If an organism has 32 chromosomes describe where/how they got that number of chromosomes.
kvv77 [185]

Answer: They got 16chromosomes from each parent

Explanation:

5 0
3 years ago
1. What are the independent and dependent variables in each of the experiments?
Dafna1 [17]
  • The independent variables are temperature, airflow, and light and the dependent variable in each of the experiments is evaporation.
  • The schematic representation in the diagram helps in the understanding of the setup for the experiments.
  • The level of temperature, the rate of airflow, and the intensity of light must be controlled in the experiments.
  • A person will not be confident in experiment 3 results as the temperature is the independent variable that alters the results of evaporation.

<h3>What are the four foremost factors of a managed test?</h3>

When possible, scientists take a look at their hypotheses through the use of managed experiments. A managed test is a systematic take look at executed below-managed conditions, which means that simply one (or a few) elements are modified at a time, even as all others are saved consistently. We'll appear carefully at managed experiments within side the subsequent section.

  • In the three experiments, the dependent variable is evaporation, as it changes with the change in another variable.

The independent variable in experiment 1 is temperature, experiment 2 is airflow, and experiment 3 is light.

  • The experiment can be understood better with the representation of the schematic that shows the setup to analyze how the experiment was performed. Therefore the diagram must add the schematic of the experiment.

  • The controlled items in the experiment are the variables that are set at the particular unit to form the result. the controlled item in experiment 1 is temperature, experiment 2 is airflow from the fan, and experiment 3 is the intensity of light.

  • The result of experiment 3 is based on the dependence on the intensity of light keeping temperature constant and controlled. If the temperature is not controlled in the experiment then it may lead to an alteration in the results as it depends on both light and temperature.

  • The flow chart for the increase in pressure affecting the evaporation is given in the image attached.

Read more about variables :

brainly.com/question/2804470

#SPJ1

6 0
2 years ago
Other questions:
  • Which of the following best describes an adaptation that developed, enabling primitive plants to make the transition to survival
    9·1 answer
  • Animal cells contain all of the following structures EXCEPT a A) nucleus. B) cell wall. C) mitochondria. D) Golgi apparatus.
    6·2 answers
  • PLEASE HELP...IM SO CONFUSED
    13·2 answers
  • Why is blood a slightly higher temperature than typical body temperature?
    7·1 answer
  • Hepatitis, HIV, and herpes are examples of _____.
    12·2 answers
  • Why does earth turn so slowly?​
    12·1 answer
  • If two organisms reproduce asexually, then they will be able to produce offspring _______ two organisms that reproduce sexually.
    6·1 answer
  • -800,000
    5·2 answers
  • The question above, I’ll reward brainalist
    10·1 answer
  • Why did scientists want to learn more about how the Bajau people hold their
    8·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!