Answer:
Since high ethanol is a major stress during ethanol fermentation, ethanol-tolerant yeast strains are highly desirable for ethanol production on an industrial scale. A technology called global transcriptional machinery engineering (gTME), which exploits a mutant SPT15 library that encodes the TATA-binding protein of Saccharomyces cerevisiae (Alper et al., 2006; Science 314: 1565-1568), appears to be a powerful tool. to create ethanol tolerant strains. However, the ability of the strains created to tolerate high ethanol content in rich media remains to be demonstrated. In this study, a similar strategy was used to obtain five strains with higher ethanol tolerance (ETS1-5) of S. cerevisiae. When comparing the global transcriptional profiles of two selected strains ETS2 and ETS3 with that of the control, 42 genes that were commonly regulated with a double change were identified. Of the 34 deletion mutants available in an inactivated gene library, 18 were sensitive to ethanol, suggesting that these genes were closely associated with tolerance to ethanol.
Explanation:
Eight of them were novel and most were functionally unknown. To establish a basis for future industrial applications, the iETS2 and iETS3 strains were created by integrating the SPT15 mutant alleles of ETS2 and ETS3 into the chromosomes, which also exhibited increased tolerance to ethanol and survival after ethanol shock in a rich medium. Fermentation with 20% glucose for 24 h in a bioreactor revealed that iETS2 and iETS3 grew better and produced approximately 25% more ethanol than a control strain. The performance and productivity of ethanol also improved substantially: 0.31 g / g and 2.6 g / L / h, respectively, for the control and 0.39 g / g and 3.2 g / L / h, respectively, for iETS2 and iETS3.
Therefore, our study demonstrates the utility of gTME in generating strains with increased tolerance to ethanol that resulted in increased ethanol production. Strains with increased tolerance to other stresses such as heat, fermentation inhibitors, osmotic pressure, etc., can be further created using gTME.
The answer should be rectum.
So it will float on top of the water
Answer:
A qualitative observation involves the use of five sensory organs, sight, smell, taste, touch, and hearing, and their function to examine the attributes. Being subjective in nature, it focuses on the characteristics and qualities of the variables rather than the numerical value.
Explanation:
mark as a brainliest answer if it helps you
Answer:
Among others, two adaptations might be
- Avoiding corporal heat loss
- Increasing oxygen absorption
Explanation:
Up in the mountains, there is low oxygen, food is scarce, and adverse meteorological conditions. Animals and plants need to develop different strategies to survive. These adaptations involve not only physical and physiological changes but also behavioral changes. To mention a few adaptations, we can name:
- Avoiding heat loss. Temperature tends to be very low at highs, so, to <u>avoid heat loss,</u> animals develop shorter legs, tails, and ears. By doing this they reduce the area or surface of heat loss and also avoid getting frozen. In mammals, the coat is also very important. A thick coat helps them maintain a constant body temperature and keep warm. Some amphibians might also develop a thicker skin as they can not regulate their temperature, and it also helps them not to dehydrate.
- Camouflage: Coat is also helpful in camouflaging. Mammals´ hair color depends on their environment. Some animals, such as hares, can also change their fur color depending on the season. During snow seasons they turn white, and during the warmer season, they turn yellow or brown.
- Size and metabolism: Small mammals lose heat very fast, so they need to keep active and feeding most of the time. They have an elevated metabolism to keep warm. On the contrary, big animals, such as bears, need to hibernate to reduce their metabolism and get to survive, otherwise, they would need many reserves to cover their energetic requirements.
- Oxygen absorption: Some animals have adapted to the lack of oxygen by increasing their heart and lungs capacity as well as their capability to absorbing more oxygen from the blood.
