Question

Suppose that a thin-layer chromatography plate the solvent front migrates a distance of 14 cm, and two steroid spots move distances of (a) 6 cm and (b) 12 cm. Calculate the Rf values for each steroid.

Answer 1

Answer:

A) $RF = 0.428$

B) $RF = 0.857$

Explanation:

RF value in thin-layer chromatography plate is defined as the ratio of distance moved by the steroid spot (A) to the distance migrated by solvent front(B)

Hence, mathematically

$RF = \frac{A}{B}$ ------Equation (A)

a) Here $A = 6$ cm and $B = 14$cm

Substituting the given values in above equation, we get -

$= \frac{6}{14} \\= 0.428$

b) Here $A = 12$ cm and $B = 14$cm

Substituting the given values in above equation, we get -

$= \frac{12}{14} \\= 0.857$