I would go with B & D because they change over time and they can go extinct like those big octopus in the oceans, they are rare to see in my opinion because they can blend in with other things that keeps them away from danger.
Answer:
Since the beginning of life of the first multicellular organisms, the preservation of a physiologic milieu for every cell in the organism has been a critical requirement. A particular range of osmolality of the body fluids is essential for the maintenance of cell volume. In humans the stability of electrolyte concentrations and their resulting osmolality in the body fluids is the consequence of complex interactions between cell membrane functions, hormonal control, thirst, and controlled kidney excretion of fluid and solutes. Knowledge of these mechanisms, of the biochemical principles of osmolality, and of the relevant situations occurring in disease is of importance to every physician. This comprehensive review summarizes the major facts on osmolality, its relation to electrolytes and other solutes, and its relevance in physiology and in disease states with a focus on dialysis-related considerations.
Answer: The simplest way is to determine if a strain is mutant is observing morphology, growth rate, double time, etc but it is accurate if you can prove if the strain is deficient in one aminoacid or can't metabolize lactose, etc.
Explanation: A wildtype strain functions normally, for example, can metabolize as a carbon source, glucose, lactose and other sugars, can synthesize all the aminoacids requered for protein synthesis, etc. If a strain suffers a mutation and it is inheritable, the strain become a mutant. Since several mutations can be silent ones, only those that interfere with a process, can be assesed easyly.
For example, if you have several strains and put them in a lactose medium, but some of them cannot growth means that are lactose mutants. Those strains could carry a mutation in genes that encode lactose degrading enzymes or in regulatory genes of the lac operon, etc.