Answer:
Ionic bonds usually occur between metal and nonmetal ions. For example, sodium (Na), a metal, and chloride (Cl), a nonmetal, form an ionic bond to make NaCl.
Explanation:
Hence, concentration of base is 1.17 M
Answer:
5.18 m x 0.77 m x 10.22 m3 = 40.763492 m^5
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Answer:
There is two common methods to separate a phosphorylated protein from the proteins who does not have the phosphate group.
1. Western Blot test
The Western blot test is a method where we separate of the biological sample with SDS- PAGE (electrophoresis method) and then we transfer to a nitrocellulose membrane. We use a phospho-specific antibody to identify the protein of interest.
2. The ELISA test
this test is also a powerful method for measuring protein phosphorylation. Elisa test is more are quantitative than Western blot test and also shows great advantage in studies related with kinase activity. In this test we use a capture antibody specific for the desired protein, independent of the phosphorylation state. The target protein is binded to the antibody-coated plate. and finally a detection antibody specific for the phosphorylation site analyzed is added. In these test it is possible to use colorimetric or fluorometric detection.
