How do trees control noise pollution?

Biology

1 answer:

klio [65]3 years ago

6 0

Answer:

A properly designed buffer of trees and shrubs can reduce noise by about five to ten decibels — or about 50 percent as perceived by the human ear, according to the USDA National Agroforestry Center

Explanation:

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A DNA fragment is introduced into the lacZ gene of a plasmid, which also contains an ampicillin resistance gene. What is the app

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Answer: The bacteria transformed with this particular plasmid will form white colonies on the plates containing ampicillin and Xgal.

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In recombinant DNA technology, when a plasmid is to be used to transform a host cell, such markers are used to help screen the transformed cells from the ones that have not taken up the plasmid. Xgal present in the plates is an artificial substrate which is hydrolyzed by

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Therefore, when a bacterial cell is transformed with a plasmid containing ampicillin resistance gene and a DNA fragment introduced in the lacZ gene and then grown on plates containing ampicillin and Xgal, white colored colonies will appear. The white colonies will show the bacterial cells that have successfully taken up the plasmid with the DNA fragment incorporated in the lacZ gene as this will render the gene non-functional and will not produce β-galactosidase which will breakdown Xgal to give blue colonies. Since the plates contain ampicillin, only the bacterial cells that have been successfully transformed with the plasmid ( the ones that have the DNA fragment and the ones without it) will grow as the ampicillin resistance will give them resistance against ampicillin in the plates. The bacterial cells that have not taken up the plasmid will not be resistant to ampicillin and will not form colonies on the plate.

This is called blue-white screening which is used to identify successfully transformed host cells. A picture of this is given in the attachment, taken from the following website:

https://www.mun.ca/biology/scarr/Blue_&_White_Colonies.html