The following are the characteristics of Cnidaria:
1. The organisms have specialized stinging cells known as cnidocytes.
2. Two types of body plans exist, that is, polyp and medusa.
3. The phenomenon of alternation of generation is observed.
The following are the characteristics of Porifera:
1. The organisms lack symmetry.
2. The adult forms are sessile.
3. The organisms obtain food by filtering water.
Answer:
The correct order is dehydration, embed in wax, cut into sections, staining
Explanation:
There are certain proceedings needed to obtain stained sections of vegetable or animal tissues for their microscope observations.
These steps are:
- Obtention of the material: The tissue is cut to an adequate size.
- Fixation: When tissues are extracted from the organism, they suffer autolysis and putrefaction, so they need to be fixated in order to keep their cells in the best state possible. Fixation involves preserving the original morphological and molecular characteristics of the tissue. Fixation avoids autolysis, putrefaction, distortion, and retraction of cells and the tissue that could affect its volume or morphology.
- Dehydration. Once fixated, the fixator must be eliminated and the tissue is dehydrated by using a gradual series of solutions with alcohol in ascendant concentrations. Dehydration must be gradual to avoid tissue deformation.
- Inclusion. To obtain thin cuts that can be observed under the optic microscope, the tissues must be included in a consistent, firm substance, that might be either hydrophilic or hydrophobic. A hydrophobic medium is paraffin wax, that provides hardness and plasticity.
- Cut. The tissue included in wax must be cut in slides or sections thin enough to allow the diffusion and penetration of light. A microtome is used to perform these cuts. When using paraffine for tissue inclusion, the cuts are about 5 to 20 micrometers of thickness.
- Stain. Once the cuts are performed, paraffin wax must be eliminated. This can be done by using an organic solvent. Then the tissue must be stained. Hematoxylin and Eosin are the most common dyes. Animal tissues in general do not have any natural color, so they need to be stained to be observed.
<span>In horses, the most distal bone in their hindlimb, which is the cannon bone is homologous to the metatarsal bones in humans.</span>
The answer is; E
This shows that these organelles were once free-living organisms with their own mechanisms for reproduction which they have retained even after years of symbiotic relationship with the higher organisms. During their duplication, they also replicate their own DNA. Their DNA does not have histones (as opposed to the DNA in the nucleus of their host eukaryotic cell) similar to that of simple organisms such as bacteria.
