Question

Correctly identify the steps and elements in this bacteriophage plaque assay

Answer 1

Answer:

The Bacteriophage:

The bacteriophage act as the parasite for most of the bacterial cells, as they attack the cells and invades its cellular boundaries to injects its DNA into the unicellular organism. The bacteriophage uses the host's resources and grows its number inside the cells body. While, most of the time there genes or strands of DNA lie dormant inside the genome of the bacterial cells, on other occasions the bacterial cell ruptures, and the newly formed phages are released into the environment.

Explanation:

Now there are few steps through which the phage passes and forms a number of its look alike in any space with the optimum amount resources.

1. Step- In the given diagram we see the mixture of molten top agar, bacterial cells and diluted phage suspension, as the first step in the process for the bacterial cells is to find the right amount resources,
2. Step- Then to start the process the cell phage mixture in poured on to an agar plate,
3. Step- The process requires some time duration or tenure to assemble its self and for that reason the mixture is left to solidify,
4. Step- Incubation allows microbial growth and phage replication,
5. Step- Phage plaques,
6. Step- Lawn of host cells

Answer 2

Step-1: Mixture of molten top agar, bacterial cells and diluted phage suspension

Step-2: Cell phage mixture in poured on to an agar plate

Step-3: Mixture is left to solidify

Step-4:  Incubation allows microbial growth and phage replication

Step-5: Phage plaques

Step-6: Lawn of host cells