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vesna_86 [32]
3 years ago
12

The purpose of a mordant in the Gram stain is to:a. remove the simple stain. b. make gram-negative cells visible. c. prevent the

crystal violet from leaving the cells. d. make the bacterial cells larger. e. make the flagella visible.
Biology
1 answer:
xenn [34]3 years ago
7 0

Answer:

c. Prevent crystal violet from leaving the cells

Explanation:

Gram Staining:

  • Gram staining is a staining techniques used for the identification of bacteria in microbiological studies.
  • Gram staining uses crystal violet as the primary stain. It complexes with the peptidoglycan cell wall of Gram positive bacteria giving them a purple color.
  • An iodine mordant is also used that forms a crystal violet-iodine complex that fixes the crystal violet dye to the cell wall. This prevents the Gram positive cell walls from being decolorized by the application of alcohol.
  • A counter stain, safranin is then applied that stains Gram negative bacteria pink.
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