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ladessa [460]
3 years ago
5

Cas9 cleaves both strands of the target DNA. Which of the following could occur when cells will try to repair the damage after c

leavage by Cas9? (Select all that apply.)mutations that result in changes to the open reading frame (ORF) of the targeted geneloss-of-function mutations within the targeted genethe insertion of a DNA sequence into the targeted geneno change in the targeted genedeletions of the targeted gene
Biology
1 answer:
Aloiza [94]3 years ago
5 0

The correct answers are: the insertion of a DNA sequence into the targeted gene and deletions of the targeted gene

Cas9 is an enzyme endonuclease (which means it cuts nucleotides) that is part of the CRISPR-Cas9 complex- genome editing tool that can remove, add or alter sections of the DNA sequence.

Cas9 recognize the sequence that is going to be cut thanks to guide RNA- pre-designed RNA sequence complementary to sequence of interest. DNA repair machinery will try to fix the “damage” but it can add (insert) or delete some nucleotides.

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Embryology provides evidence for evolution because
Free_Kalibri [48]
The answer would most likely be D. During their development, many organisms look similar, suggesting that very different organisms may have a common ancestor.

This is because this be evidence to the theory of common decent
5 0
4 years ago
Read 2 more answers
If I wanted to test whole milk using all of the indicators (biuret, benedicts, iodine and brown paper bag), what results would y
stellarik [79]

Whole milk will give a biuret positive, Benedict positive, iodine negative and brown paper bag test positive results.

<h3><u>Explanation:</u></h3>

Whole milk is obtained from mainly the dairy animals like cow, Buffalo etc. It contains different ingredients of diet and is considered as an ideal balanced diet for infants.

The Benedict's test is a test to identify the presence of reducing sugars. Milk contains reducing sugar lactose. This is why milk gives Benedict's positive result.

Biuret test also gives positive result because it is a test for protein and milk contains enough of this. The protein in milk is called casein.

The brown paper bag test is a test for lipid and its positive because the milk contains enough lipid. It depends on milk source.

The iodine test is a test for starch and milk isn't a good source of starch. So iodine test isn't positive.

8 0
3 years ago
In an ape, the space between the upper lateral incisor and the canine that accommodates a large, projecting lower canine is a:
iVinArrow [24]

Answer: diastema

Explanation:

Diastema is condition in which a gap develops in between the two teeth. The spaces or gaps develop in the upper front teeth. The space or gap may appear between the biting teeth (canines or incisors) and in between the grinding teeth (molars or premolars). This occurs when there is unequal relationship develop between the size of jaw and teeth. The space can be too large that it can accommodate the parts of lower canine.

According to the above description, the condition can be considered as diastema.

3 0
4 years ago
Nucleotide hydrolysis dehydration monosaccharide polypeptide amino acid polysaccharide polynucleotide
melamori03 [73]

Answer:

The answer is explained below;

Explanation:

  • Dehydration - A reaction builds polymers from monomers.

Macromolecules such as carbohydrates, proteins, lipids, nucleic acids, etc are made by joining small molecules (monomers). Dehydration is a process which combines monomers by covalent bonds to form polymers by releasing a molecule of water. This reaction requires energy. Here, the release of a water molecule occurs when two hydrogen atoms from one monomer combine with one oxygen from the other monomer or the hydrogen of one monomer combines with the hydroxyl group of another monomer. Proteins, nucleic acids such as DNA, etc examples of polymers formed by dehydration reaction.  

  • Hydrolysis- A reaction breaks down polymers into monomers.

Hydrolysis is a process which breaks down polymers into monomers by adding a molecule of water. This reaction release energy by breaking the bonds. When the polymer is broken into two components, one component receives a hydrogen atom and the other obtains a hydroxyl group from a water molecule. The digestion process of macromolecules like carbohydrate is an example of hydrolysis reaction.

  • Nucleotide - A building block of polynucleotides such as DNA

It is an organic molecule that functions as the building block of polynucleotides such as DNA and RNA. It consists of a nucleoside (a nitrogenous base or nucleobase and a five-carbon sugar- ribose or deoxyribose) and a phosphate group. Nucleotides have roles in cell signaling, metabolism and energy production in cells, enzymatic reactions, etc.

  • Polynucleotide - a polymer of nucleotides.

It is a biopolymer molecule consists of many nucleotide monomers that are covalently bonded to form a chain. The examples of polynucleotides  are DNA (deoxyribonucleic acid) and RNA (ribonucleic acid) in which four nitrogenous bases are present i.e., in DNA they are guanine, adenine, cytosine and thymine and in RNA, uracil is used instead of thymine.  

  • Amino acid - a building block of polypeptides, such as the protein hemoglobin.

It is an organic molecule contains a basic amino group, an acidic carboxyl group, and an organic side chain (R group). The side chain is unique to each amino acid. Amino acids are the building blocks of polypeptides (proteins) and help in the synthesis of hormones and neurotransmitters.

  • Polypeptide - a polymer of amino acids.

It is a short unbranched chain of amino acid sequence linked together by peptide bonds between the amino and carboxyl groups of adjacent amino acids. The different peptides are dipeptides, tripeptides, tetrapeptides, etc and proteins are made by one or more polypeptide chains that support the cell structure.

  • Monosaccharide - a building block of polysaccharides such as starch.

It is the simplest form of sugar which consists of carbon, hydrogen, and oxygen atoms and cannot be broken down further. It is the building block of polysaccharides and includes aldoses, ketoses, etc.  

  • Polysaccharide - a polymer of monosaccharides.

Polysaccharides (oligosaccharides) are long-chain polymers formed by joining of monosaccharides together by glycosidic bonds. The examples of polysaccharides are cellulose (structural polysaccharide in plants), glycogen (the storage form of glucose in the human liver and muscles), starch (energy source obtained from plants), etc.

7 0
4 years ago
The table shows a mutation in a DNA sequence.
Temka [501]

its type of mutation depicted in the table is D.point

5 0
3 years ago
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