Question

Cas9 cleaves both strands of the target DNA. Which of the following could occur when cells will try to repair the damage after cleavage by Cas9? (Select all that apply.)mutations that result in changes to the open reading frame (ORF) of the targeted geneloss-of-function mutations within the targeted genethe insertion of a DNA sequence into the targeted geneno change in the targeted genedeletions of the targeted gene

Answer 1

The correct answers are: the insertion of a DNA sequence into the targeted gene and deletions of the targeted gene

Cas9 is an enzyme endonuclease (which means it cuts nucleotides) that is part of the CRISPR-Cas9 complex- genome editing tool that can remove, add or alter sections of the DNA sequence.

Cas9 recognize the sequence that is going to be cut thanks to guide RNA- pre-designed RNA sequence complementary to sequence of interest. DNA repair machinery will try to fix the “damage” but it can add (insert) or delete some nucleotides.