When cloning by restriction digest and ligation, you use restriction enzymes to cut open a plasmid (backbone) and insert a linear fragment of DNA (insert) that has been cut by compatible restriction enzymes. An enzyme, DNA ligase, then covalently binds the plasmid to the new fragment thereby generating a complete, circular plasmid that can be easily maintained in a variety of biological systems. Read on for an in-depth breakdown of how to do perform restriction digests.
PMI is very important in forensics because having a time of death can help with the identification of human remains and can support or deny the stated actions of suspects in the crime.
Answer:
The answer is one cell.
Mitosis is a cell division of a single cell into two identical daughter cells. So, at the beginning of mitosis, there is only one cell. It will undergo different stages of mitosis (prophase, metaphase, anaphase, telophase and cytokinesis) which will result in the formation of two daughter cells that are identical to the mother cells.
Explanation:
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The scientist should notice that the cancer cells have a <u>higher</u> rate of cell division and have a <u>lower</u> response to inhibiting factors. Cell groups that are cancerous should have a <u>lower</u> percent of cells in interphase than noncancerous cells.
Explanation:
The general characteristics of cancerous cells are defined by the ‘hallmarks of cancer’ like ‘enable replicative immortality, activate invasion and metastasis, resist cell death, evade growth suppressors’ etc. All these facts prove that they grow and divide at a higher rate and undergo uncontrollable mitotic cell division leading to proliferative invasion.
The cancerous cells also show low or no response to inhibiting factors like growth suppressors like p53, cyclins and other anti-tumor drugs.
The number of cancer cells in interphase is lower than noncancerous cells because interphase is a phase of cell growth, development and differentiation. Cancer cells undergo uncontrollable mitosis and more time is spent after the interphase to facilitate rapid cell division rather than growth or development.
Answer:
-3
Explanation:
because when you do this math solution you get -3
