An interesting enzyme, whose molecular weight is 56,000 g/mol, is used in catalytic amounts to cleave a membrane protein, whose
molecular weight is 65,000 g/mol, into two fragments having molecular weights of 43,000 and 22,000 g/mol. Gel chromatography is used to assess the extent of reaction at various times. Three major fractions of proteins are eluted at each time. What are they and in what order are they eluted
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<h3>Answer:</h3>
16.7 g H₂O
<h3>General Formulas and Concepts:</h3><u>Math</u>
<u>Pre-Algebra</u>
Order of Operations: BPEMDAS
- Brackets
- Parenthesis
- Exponents
- Multiplication
- Division
- Addition
- Subtraction
- Left to Right
<u>Chemistry</u>
<u>Stoichiometry</u>
- Reading a Periodic Table
- Using Dimensional Analysis
<u>Step 1: Define</u>
[RxN - Balanced] 2NaOH (s) + CO₂ (g) → Na₂CO₃ (s) + H₂O (l)
[Given] 1.85 mol NaOH
<u>Step 2: Identify Conversions</u>
[RxN] 2 mol NaOH → 1 mol H₂O
Molar Mass of H - 1.01 g/mol
Molar Mass of O - 16.00 g/mol
Molar Mass of H₂O - 2(1.01) + 16.00 = 18.02 g/mol
<u>Step 3: Stoichiometry</u>
- Set up:
- Multiply/Divide:
<u>Step 4: Check</u>
<em>Follow sig fig rules and round. We are given 3 sig figs.</em>
16.6685 g H₂O ≈ 16.7 g H₂O