1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
natima [27]
2 years ago
8

Why are there nucleotides (A, T, G, and C) in the master mix? What are the other components of the master mix, and what are thei

r functions?
Biology
1 answer:
tankabanditka [31]2 years ago
8 0

Answer: Nucleotides are the monomers used for DNA synthesis. The mix also contains a template, DNA taq polymerase, buffer, reverse and foward primer and magnesium ions.

Explanation:

A PCR master mix is a premixed solution that has all of the components for a Polymerase chain reaction (PCR). This reaction is a laboratory technique that amplifies small fragments of DNA into millions of copies.

The master mix used for that contains dNTPs (nucleotides). In the DNA there are four types of nucleotides that are differentiated by the nitrogen base they have: adenine (A), guanine (G), cytosine (C) and thymine (T).

<u>Since nucleotides are the monomers that make DNA, they are found in the mix because they are the material for DNA synthesis.</u>

The reaction mixture also requires;

  • DNA template, the sequence of DNA to be amplified
  • DNA taq polymerase, a heat resistant enzyme that assembles nucleotides into a new DNA
  • Salt buffer, for an optimum ionic environment and pH
  • Oligonucleotide primers (reverse and foward), pieces of DNA complementary to the template. Each hybridizes with one of the DNA chains.
  • Magnesium ions, a catalyst required by DNA polymerase to work
You might be interested in
___________ claims are initiated by the parent and/or child based on the harm suffered as a result of being born.
aleksley [76]

Answer:

Wrongful life

Explanation:

Wrongful life is the term used for the claim initiated by the parent based on the harm or injury brought upon a child at birth either due to negligence of the health caregiver or health facilities

4 0
3 years ago
Help, i have info there and i need the punnet square filled! plz
maxonik [38]

Answer:

X.       x

Y /. XY      / Yx

----------

y /.  Xx    / xy

Explanation:

hope this helps

6 0
2 years ago
What organelles can only be found in animal cells?
SOVA2 [1]
The lysosomes are the “garbage disposal” of animal cells , the same process takes place in plant cells in its vacuoles
5 0
3 years ago
PLSSSSSSSSSSSSSSSSSSSS HELP I'LL GET AN F
nexus9112 [7]

They have longer periods of revolution.

5 0
2 years ago
Read 2 more answers
The first step of the design process is to
Mekhanik [1.2K]

Answer::::::::::::::::::::C

5 0
3 years ago
Other questions:
  • Deepest place in the seafloor is called the
    13·2 answers
  • What is the difference between casual observers and scientific observers?
    8·2 answers
  • Where do the reactions of the electron transport chain occur?
    7·2 answers
  • How does DNA help with the transfer of genetic material from parents to offspring?
    11·1 answer
  • A popular pesticide used in both world wars was what
    9·1 answer
  • What crops are grown in france<br><br> Help!!
    8·1 answer
  • Why are sinkholes one major environmental risk of underground mining? (1 point)
    9·1 answer
  • Can someone help me on this please?
    6·1 answer
  • What is an example of biotechnology? What example from the Gene Editing article explains how biotechnology can be used for carbo
    14·1 answer
  • What are the nitrates is used to make?
    9·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!