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natima [27]
3 years ago
8

Why are there nucleotides (A, T, G, and C) in the master mix? What are the other components of the master mix, and what are thei

r functions?
Biology
1 answer:
tankabanditka [31]3 years ago
8 0

Answer: Nucleotides are the monomers used for DNA synthesis. The mix also contains a template, DNA taq polymerase, buffer, reverse and foward primer and magnesium ions.

Explanation:

A PCR master mix is a premixed solution that has all of the components for a Polymerase chain reaction (PCR). This reaction is a laboratory technique that amplifies small fragments of DNA into millions of copies.

The master mix used for that contains dNTPs (nucleotides). In the DNA there are four types of nucleotides that are differentiated by the nitrogen base they have: adenine (A), guanine (G), cytosine (C) and thymine (T).

<u>Since nucleotides are the monomers that make DNA, they are found in the mix because they are the material for DNA synthesis.</u>

The reaction mixture also requires;

  • DNA template, the sequence of DNA to be amplified
  • DNA taq polymerase, a heat resistant enzyme that assembles nucleotides into a new DNA
  • Salt buffer, for an optimum ionic environment and pH
  • Oligonucleotide primers (reverse and foward), pieces of DNA complementary to the template. Each hybridizes with one of the DNA chains.
  • Magnesium ions, a catalyst required by DNA polymerase to work
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Answer:

see in the picture below

Explanation:

A food chain is a flow of energy from a green plant (producer) to an animal (consumer) and to another animal (another consumer) and so on. In this lesson we are going to talk all about food chains and food webs in the environment.

8 0
3 years ago
Which list places the layers of the sun in the correct order from outermost to innermost?
mr Goodwill [35]

Answer:

The Sun's layers consist of the following in this order.

1) Corona

2) Transition Region

3) Chromosphere

4) Photosphere

5) Convection Zone

6) Radiative Zone

and last but not least 7) The Core

Hope this helps ;)

3 0
3 years ago
Read 2 more answers
How will the change in pH impact the functioning of cells? (Hint: Explain which biomolecules and what types of bonds in these bi
Effectus [21]

Answer:

A change in pH will cause many cellular processes to be disrupted because they affect the biomolecules (protein and nucleic acid) responsible for these processes.

Explanation:

pH refers to the degree of acidity or alkalinity. In a cell, the structures and processes that occur in them are impossible without the biomolecules, which are carbohydrate, protein, lipids and protein.

However, unfavorable conditions like a change in pH can cause the structure of some of these biomolecules to be affected. Proteins are made up of amino acids, which gives them their shape that is peculiar to their functioning. Also, nucleic acids such as DNA are composed of nucleotides responsible for their functioning.

A change in pH will cause the bonds of the protein to be disrupted, hence altering its shape and ultimately its functioning. Likewise, the hydrogen bonds in the DNA will be broken in the presence of a high pH causing the DNA to be dysfunctional.

When these biomolecules are affected, the vital functions that they perform in a cell, which is key to the cell's survival are disrupted) are likewise affected. Therefore, the cell is affected negatively.

8 0
3 years ago
DNA replication is Group of answer choices conservative. a one-step process. semi-conservative. not carried out by enzymes. not
Alex787 [66]

Answer:

The correct answer is: semi-conservative.

Explanation:

DNA replication is an important process in which the DNA is duplicated before cell division so each daughter cell can have a copy of genetic material.

DNA replication is <u>semi-conservative because one strand of DNA is new and the other one is the old strand</u>. To replicate itself, the DNA double helix is opened, and then each strand serves as a model for the new strands.

DNA replication is <u>not a one-step process</u>, as it takes several complicated steps in which <u>lots on enzymes participate</u> (like DNA polymerase and DNA helicase).

DNA replication is <u>also carried by prokaryotic cells</u>, though it's a much simpler process.

5 0
3 years ago
The table shows the distance between the moon and
Lunna [17]

January 10. 360,048.

Explanation:

The Earth and the Moon are two space bodies that have each other as the closest to one another. Both of them have their own gravitational pull, with the one of the Earth being much greater, while the one of the Moon being weaker than it is expected for a body of its size. Nevertheless they influence each other with the gravitational pull, which is most noticeable on Earth.

The gravitational pull between these two space bodies is the greatest when they are the closest to each other, while it is the smallest when they are the furthest from each other. On January 10 the distance between Earth and the Moon is the smallest and it is 360,048 km, so the gravitational pull is the greatest on this date. On December 19 the distance between Earth and the Moon is the greatest and it is 406,276 km, so the gravitational pull is the smallest.

8 0
3 years ago
Read 2 more answers
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