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Ymorist [56]
3 years ago
15

You transform an E. coli bacterium with the pGLO plasmid. After growing the +pGLO bacteria on an LB+Ampicillin+Arabinose plate,

you see distinct colonies form after 16 hours. You check these colonies under the UV light and see that they are glowing a bright fluorescent green color. If you use a sterile loop and inoculate a new plate with LB+Ampicillin (coated on them, as usual) with these transformed E. coli will you see any growth? If there is growth, will it glow?
Biology
1 answer:
soldi70 [24.7K]3 years ago
4 0

Answer:

You will see growth in LB+Ampicillin, but no glow

Explanation:

Expression of beta lactamase (gene that confers resistance to ampicillin) is constitutive in pGLO, but expression of GFP is dependent on the presence of arabinose

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