Answer:
DNA digestion was not complete.
Explanation:
When a particular DNA needs to be used for the experiment, it is firstly processed by restriction digest. A restriction digest is a procedure for DNA preparation for further examination and manipulation. It is defined as a technique used for separating DNA fragments. This separation is being done using specific enzymes that will bond to specific sites and locations to the DNA so all DNA fragments will be a similar size. These enzymes are called restriction endonucleases or restriction enzymes and are extracted from bacteria for this particular cause. Once fractured or digested, DNA fragments may be amplified by PCR (polymerase chain reaction) and used for further experimentation.
When DNA is sequenced, all recognition sites (on enzymes) are visible and detectable. However, if an extra recognition site is found after sequencing, it means that initial mapping was not completed (assuming the DNA sequence has no errors). Initial restriction digestion was not completely done, so DNA is stuck in the middle of the process, in various degrees of supercoiling and digestion.
They must be separated to the point that their genetic makeup changes so that they can no longer breed.
It’s the remains of a dead organism
Question: What do you think high blood pressure over extended periods of time can do to such walls?
Final Answer:
Overtime with having high blood pressure. It can damage your blood vessels. With having high blood pressure it is straining your vessels. Also, with it constantly being high you are more likely to develop health problems. Some problems could be:
stroke, heart attack, and kidney damage.
Blood pressure is measured only when the person is at rest. This is best because they will get a more accurate reading.
