When cloning by restriction digest and ligation, you use restriction enzymes to cut open a plasmid (backbone) and insert a linear fragment of DNA (insert) that has been cut by compatible restriction enzymes. An enzyme, DNA ligase, then covalently binds the plasmid to the new fragment thereby generating a complete, circular plasmid that can be easily maintained in a variety of biological systems. Read on for an in-depth breakdown of how to do perform restriction digests.
There are five classes of antibody.
1) <span>IgA (immunoglobin A)
2) IgD (immunoglobin D)
3) IgE (immunoglobin E)
4) IgG (immunoglobin G)
<span>5) IgM (immunoglobin M)
They form Y shaped antibodies. The crystallized fragment is the stalk of the Y shaped antibodies. The bond formed by this stalk will determine the form of the antibody. One Y is called monomer, two connected Ys are called dimers, and 5 connected Ys are called pentamers.</span></span>
Graphs that are clearly labeled are much less likely to be misread or misinterpreted.
Answer:
i think they are orignally from cold areas so they store water and moisture in there needles. and they stay green year round.
Explanation:
1.An open mind
2.Curiosity
3.Flexibility
4.Creativity
5.Patience and
6.Observation skills
