I would say 4. To create a hypothesis, you would need some knowledge about what you're studying. This is where prior knowledge could come in handy.
soil, rock, organic layer
Explanation:
Permeability is the ability of a layer of rock to transmit fluid such as oil or water
The factors that affect the permeability of a rock layer includes the sizes of the rock particles, the ratio of the available voids to the solid mass of the rock, the presence of trapped air and the presence of organic matter
Rocks such as gravels, and sparingly cemented sands have high permeability
The most impermeable of the options are granite and clay which for granite has large particle mass and contain no voids while clay has very fine particles packed together with little room for water
Therefore, water moves easily between layers soil, rock, organic layer
It is thigmotropism because it is not affected by gravity or light.
In terms of macromolecules, needed and utilized by organisms to carry out essential metabolic chemical reactions and for maintaining structure would be Lipids.
Lipid based molecules or Fats tend to store the most energy, as breaking chemical bonds connecting fatty acid chains of long hydrocarbons releases quite a bit of energy.
