Answer:
Given:
Number of colonies on plate = 40
Amount of DNA used for transformation = 100ng
Volume of competent cells used = 100ul
Total volume = 1ml (1000ul)
Volume plated for growth = 200ul
The amount of cells transformed per 1 µg of DNA is called the transformation efficiency
(CFU is colony forming units) => (number of colonies on plate/ng of DNA plated) X 1000 ng/µg = CFU/µg of DNA
Amount of DNA used for plating = 100ng * (200/1000)
= 100ng * (1/5)
= 100/5 = 20ng
Efficiency calculation = 40 colonies / 20ng = 2 colonies/ng
(2 colonies/ng) * (1000 ng/µg) = 2000 CFU/ug DNA
Transformation efficiency = 2000 Transformants / ug DNA
= 2 x 10³ Transformants / ug DNA
Explanation:
Efficiency of transformation is highest in the 100 pg-1 ng range, so Low transformation efficiency could be because of high amount of DNA used for transformation.
Answer: Thalamus
Explanation:
The thalamus part of the brain is known as the relay station for all the sensory information. This information can be any sought of smell, light, taste et cetera.
It is relayed further via thalamus. Myelinated fiber bundle originates from the lateral surface of thalamus and it terminates at the cerebral cortex.
It is a relay station for the information from brainstem, spinal cord and cerebrum towards the cortex.
<span>The correct answer is that he has injured one of the tendons or ligaments that help keep his knee in place, connecting it to the surrounding muscle and bone. As a result of this injury, his knee is no longer connected to the muscle and bone, thus it wobbled sideways when he started walking. He needs to go to a doctor immediately who can fix his knee so as to prevent further complications - he just needs his knee to be set in place again.</span>
Answer:
The basic repeating unit of nucleic acids are known as nucleotides. A nucleotide consists of three distinct chemical groups, a 5-carbon sugar (ribose or deoxyribose), a nitrogen-rich base - (cytosine (C), guanine (G), adenine (A), thymine (T) in DNA or uracil (U) instead of T (in RNA), and phosphate.
Isolated on its own, bloodplasma is a light yellow liquid, similar to the color of straw. Along with water,plasma carries salts and enzymes. The primary purpose of plasma is to transport nutrients, hormones, and proteins to the parts of the body that need it.
Hemoglobin is the protein inside red blood cells that carries oxygen. Red blood cells also remove carbon dioxide from your body, transporting it to the lungs for you to exhale. Red blood cells are made inside your bones, in the bone marrow. They typically live for about 120 days, and then they die.
White blood cell. ... White blood cells(also called leukocytes or leucocytes and abbreviated as WBCs) are thecells of the immune system that are involved in protecting the body against both infectious disease and foreign invaders.
Platelets are tiny blood cells that help your body form clots to stop bleeding. If one of your blood vessels gets damaged, it sends out signals that arepicked up by platelets. The plateletsthen rush to the site of damage and form a plug, or clot, to repair the damage.
