Autoclaving is a sterilization method that involves the use of high temperature and pressure. In medical application, it is used for the sterilization of medical equipment and also used for vulcanizing rubber and curing coatings in the chemical industry.
Autoclaving process destroys living cells as well as bacterialendospores. Endospores are tough non reproductive structures produced by some bacteria. They have the ability to be dormant, hence can enable bacteria to survive environmental stress.
Autoclaving is a sterilization method that is used to sterilize nutrient media,laboratory materials and uses high pressure to kill infectious agents and denature protein. It works base on the fact that the boiling point of water increases under high pressure. Autoclaving destroys living cells and endospores of bacteria. The endospores of bacteria are hard or tough in reproductive part.