Answer:
Air, water life without these is nothing
The proteins move through the endomembrane system and are dispatched from the trans face of the Golgi apparatus in transport vesicles that move through the cytoplasm and then fuse with the plasma membrane releasing the protein to the outside of the cell.
The large central vacuole would become dehydrated, because without enough water stored in it, there wouldn't be any turgor pressure (water pushing against the cell wall) to support the cell, which causes the plant to wilt.
After looking at the diagram of organism, the sequence that will best represent the food chain within this particular food web is sedges-ants-frogs-kookaburras. So the correct answer choice will be number 2.