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MA_775_DIABLO [31]
3 years ago
12

A sample of ocean water is tested for its bacterial content in a plate count assay. A one-milliliter sample of the water is dilu

ted in a 1:10 dilution series. 0.1 milliliter of the second dilution tube is plated in a pour plate. After incubation, the plate has 191 colonies, indicating that the original water sample contained______________
Biology
1 answer:
Alja [10]3 years ago
7 0

Answer:

191000 cells per millilitre

Explanation:

After incubation the plate has 191 colonies. Since each colony grows from a single cell, the plate had 191 cells.

Now only 0.1 millilitre was plated from 1 millilitre of sample hence number of cells in 1 millilitre of sample is 10 * 191 = 1910 cells.

It has been given that this sample is taken from second dilution tube of 1:10 dilution series. So total dilution here has been of 100 times. Hence, original water sample contained 1910 * 100 = 191000 cells per millilitre.

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The pyruvate dehydrogenase complex catalyzes the oxidative decarboxylation of pyruvate to form acetyl‑CoA. E 1 , E 2 , and E 3 a
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Answer:

E1: Pyruvate dehydrogenase, TPP, oxidative decarboxylation reaction

E2: Dihydrolipoyl transacetylase, Lipoamide and Co-enzyme A, transacetylation reaction.

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Explanation:

Pyruvate dehydrogenase is a multi-enzyme complex with 5 co-enzymes and 3 apo-enzymes:

Pyruvate dehydrogenase (E1) , which uses thiamine pyrophosphate (TPP) as as co-enzymes to catalyze oxidative decarboxylation of pyruvate to hydroxyethyl-TPP.

Dihydrolipoyl transacetylase (E2): which uses lipoamide and coenzyme A as co-enzymes to catalyse the transacetylation from TPP to Lipoamide to form acetyl lipoamide.

Dihydrolipoyl dehydrogenase (E3)​​ which uses FAD and NAD+ as co-enzymes to catalyze the oxidation of lipoamide

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3 years ago
Why is pyruvic acid never the end product of fermentation
Igoryamba

Answer:

Pyruvic acid is not the end product of fermentation because in fermentation the pyruvic acid is converted into alcohol/lactic acid and carbon dioxide. There are two types of fermentation alcoholic fermentation and lactic acid fermentation.

In alcoholic fermentation, pyruvate is converted into ethanol and CO2 for example in yeast and in lactic acid fermentation pyruvate is converted into lactic acid and CO2 for example in animals muscles.

Therefore in fermentation pyruvic acid is not the end product of fermentation. In fermentation 2 ATP is produced.  

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